13-15 December 2023.

We will introduce to you our cell culture laboratory where we are working with in vitro models of biological barrier systems. We will demonstrate which model is used as a drug entry gate and how we study the effects of drugs on cells, their entry into the cell and their translocation through the barrier system model.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62., 1. emelet 148-as labor)

SZENT-GYÖRGYI MENTOR:

Mária Deli

Our research group focuses on image-based single-cell analysis to study tumor heterogeneity. First, our AI-driven algorithms analyze high-resolution whole slide images to detect the cells and explore different tumor cell types using morphological information. These different tumor cell types will be isolated by laser-microdissection to perform multi-omic measurements. Finally, the huge multi-omic dataset will be analyzed to identify molecular changes at the phenotypic level.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62.)

SZENT-GYÖRGYI MENTOR:

Péter Horváth

Antibiotic resistance nowadays and its illustration through the research work of the group.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62.)

SZENT-GYÖRGYI MENTOR:

Csaba Pál

We introduce the genetic, microscopic and molecular biology toolkit of the fruitfly (Drosophila melanogaster) model in the context of autophagy (cellular self-digestion), a Nobel award-worthy topic.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62., 5. emelet, 535-ös labor (Izotóp szárny))

SZENT-GYÖRGYI MENTOR:

Gábor Juhász

We will demonstrate the structure and functions of brain blood vessels using different fluorescent microscopic methods (i.e. two-photon microscopy, STED super-resolution microscopy). Some topics we are planning to cover include the blood-brain barrier, inflammatory processess in the central nervous system and in ageing, the regeneration of microvessels with progenitor cells and the formation of brain metastases.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62.)

SZENT-GYÖRGYI MENTOR:

István Krizbai

Imola Wilhelm

Introduction to the Drosophila muscle models. Visit of a fly lab, examination of fluorescent streomicroscopic and confocal microscopic samples.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62.)

SZENT-GYÖRGYI MENTOR:

József Mihály

Metabolomics allows us to observe what metabolites are present in the body and in what quantities. This can help to identify the causes of a disease and to develop more effective treatments. It is, therefore, an important tool for the future of medicine. During the presentation, students will be given a brief theoretical and practical introduction to modern metabolomics techniques in our laboratory.

LOCATION:

Biological Research Centre, Szeged  
(6726 Szeged, Temesvári krt. 62.)

SZENT-GYÖRGYI MENTOR:

Balázs Papp

Microscopic examination of fruit fly brains, identification of fly strains

LOCATION:

Szeged Biological Research Centre Department of Genetics  
(6726 Szeged, Temesvári krt. 62.)

SZENT-GYÖRGYI MENTOR:

Áron Szabó

Particlesize deacresing, nanoparticle characterizations, dosageform investigations

LOCATION:

University of Szeged Faculty of Pharmacy  
(6720 Szeged, Eötvös utca 6., 3. emelet)

SZENT-GYÖRGYI MENTOR:

Rita Ambrus

Echocardiography of intact and infarcted rats to show difference in cardiac function

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School the Dept. of Pharmacology and Pharmacotherapy  
(6720 Szeged, Dóm tér 12, 4. emelet)

SZENT-GYÖRGYI MENTOR:

Péter Bencsik

Demonstration of isolated heart perfusion. Examination of the heart by cardiac ultrasound.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School the Department of Biochemistry  
(6720 Szeged, Dóm tér 9., II. emelet)

SZENT-GYÖRGYI MENTOR:

Tamás Csont

Gastrointestinal microcirculation studies by monitoring exhaled methane in a rat model - determination of mitochondrial oxygen consumption, in vitro. Exhaled methane level will measure of anesthetized and intubated SPRD rats. Parallel with methane monitoring, we measure the mean arterial pressure with a non-invasive technique on the tail. After the occlusion of mesenteric artery we examine the microcirculation of the small intestine - the results are compared with the exhaled methane level. Mitochondrial function, i.e. oxygen consumption of the electron transport chain, is determined by high-resolution fluorespirometry (Oroboros O2K oxygraph) from the left lateral lobe of the rat liver. After tissue biopsy, the liver sample is homogenized (Potter-Elvehjem), the homogenate is pipetted into the oxygraph chamber and the respiration chambers are closed with a stopper. After stable basal respiration, the sample is stimulated with succinate and ADP in two steps at 37°C with constant magnetic stirring. Mitochondrial respiration is inhibited by antimycin A (complex III inhibitor) addition.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School Department of Surgical Research  
(6720 Szeged, Szőkefalvi-Nagy Béla utca 6., III. emelet)

SZENT-GYÖRGYI MENTOR:

Mihály Boros

Isolation of neutrophil leukocytes from blood of septic rats; Detection of neutrophil extracellular trap formation by fluorescent staining; Demonstration of healthy and "diseased" neutrophil mitochondrial respiration

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School Department of Surgical Research  
(6720 Szeged, Szőkefalvi-Nagy Béla utca 6., III. emelet)

SZENT-GYÖRGYI MENTOR:

József Kaszaki

In the biomedical research novel model systems have been developed to model human diseases. Our laboratory has gained considerable experience with organoid cultures in recent years. These complex cell cultures are utilized in different research applications. Candidates will gain an understanding of the importance of organoids, their experimental use and the limitations of the system.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical Schoolt  
(6720 Szeged, Dóm tér 10.)

SZENT-GYÖRGYI MENTOR:

József Maléth

Theoretical background: The prevalence of chronic heart failure is increasing worldwide. Potential causes of chronic heart failure include, e.g. hypertension, diabetes mellitus, and chronic kidney disease. In the early phase of chronic heart failure, the left ventricular wall often thickens (i.e., hypertrophy) and develops relaxation abnormalities (i.e., diastolic dysfunction), and in the later stages, the ventricular walls become thinner, the cavities dilate, and the contractility of the heart decreases (i.e., systolic dysfunction) due to remodeling by scar tissue (i.e., fibrosis) of the heart. However, the underlying molecular mechanisms in the development of chronic heart failure are not fully understood in different co-morbidities. Therefore, the experimental investigation of the molecular mechanisms of chronic heart failure, the identification of its early predictors, the research for new diagnostic markers, prevention, and treatment with new drugs are important and clinically relevant areas. In our lab, we perform functional, morphological, and molecular studies to assess the development of chronic heart failure in rat models. Our aim is to detect molecular abnormalities related to morphologic and/or functional changes and test new agents for the prevention of ventricular wall remodeling and for the treatment of chronic heart failure. Content of the demonstrative program: The program will include a short lecture on chronic heart failure and its experimental modeling in rodent models. In addition, a demonstrative echocardiography examination will be performed on a rat, and the echocardiographic images will be analyzed. The program will take place in two rounds.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School Department of Pathophysiology  
(6720 Szeged, Szőkefalvi-Nagy Béla utca 6., II. emelet, 48. terem)

SZENT-GYÖRGYI MENTOR:

Márta Sárközy

Experiments: 1.Presentation of Ambitus test applied for the investigation of learning abilities of rats. 2. Demonstration of startle reaction for the investigation of sensory gating. 3. Displaying the rat behaviors in an automated complex cage. 4. Exploration of different brain regions for in vitro studies. Topic of presentation: Schizophrenia is a multifaceted neuropsychiatric disease with genetic and environmental backgrounds. Therefore, our laboratory developed a new, complex schizophrenia rat model (named Wisket) by combining environmental (postweaning isolation), pharmacologic treatment and genetic (selective breeding) manipulations. This presentation displays several new methods for the behavioral characterization of the impairments in the Wisket animals. In vitro studies are important tool for revealing the abnormalities in neurotransmitter systems in different brain regions, which may be related to the behavioral malfunctions.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School Department of Physiology  
(6720 Szeged, Dóm tér 10.)

SZENT-GYÖRGYI MENTOR:

Gyöngyi Horváth

Introducing the path of pathological tissue samples which can be used for molecular tumor diagnostics. Data management and bioinformatics analysis of large examination cohort samples.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School Department of Pathology  
(6725 Szeged, Állomás utca 1., 1. emelet)

SZENT-GYÖRGYI MENTOR:

Tibor Pankotai

Within the framework of the program, it will be possible to gain an insight into the daily work of the Cell Biology Laboratory of the Department of Dermatology and Allergology. Within this, participants can learn about techniques that can be used to make our cells and tissues visible. In addition to the description of the techniques, we also plan to present specific examples, for which the participants can examine cell and tissue samples of skin origin with their own eyes under a microscope.

LOCATION:

University of Szeged Albert Szent-Györgyi Medical School of Dermatology and Allergology  
(6720 Szeged, Korányi fasor 6., 3. emelet, Sejtbiológiai Labor)

SZENT-GYÖRGYI MENTOR:

Zsuzsanna Bata-Csörgő

Renáta Bozó

Examining tumor-fungal pathogen interactions: migration analysis of oral squamous cell carcinoma cells during fungal infection.

LOCATION:

University of Szeged, Faculty of Science and Informatics  
(6726 Szeged, Közép fasor 52, 3. emelet, 301-es labor)

SZENT-GYÖRGYI MENTOR:

Renáta Tóth

Examination of tumour cells exposed to various agents, cell biological changes, changes in gene expression.

LOCATION:

University of Szeged, Faculty of Science and Informatics  
(6726 Szeged, Közép fasor 52, 3. emelet, 301-es labor)

SZENT-GYÖRGYI MENTOR:

Mónika Kiricsi